Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
FUJIFILM BIOSCIENCES INC MEM 1X Earles Salts w/o NEAA
MEM 1X Earles Salts w/o NEAA is an uncomplicated medium that is useful for a wide range of species and cells
Apexbio Technology LLC Annexin V-PE/DAPI Apoptosis Kit 20tests
Annexin V-PE/DAPI Apoptosis Kit enables differentiation between early apoptotic late apoptotic and dead cells Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane phospholipid bilayer However during the early stages of apoptosis PS moves from the inner surface to the outer surface of the cell membrane becoming exposed to the extracellular environment Labeled with a fluorescence dye such as PE Annexcin V is commonly used as a fluorescent probe to detect apoptosis through flow cytometry or fluorescence microscopy within 15-30 minutes Additionally this kit includes the nuclear stain DAPI which specifically binds to double-stranded DNA to emit blue fluorescence Normally DAPI cannot penetrate intact cell membranes In late apoptotic and dead cells DAPI can cross the compromised membrane to stain the nucleus
Teknova 20X PBS with 1% Tween-20. 500mL, Sterile. 8 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Quality Biological Inc PBS pH 7.4 without Calcium and Magnesium, 4 Liters
PBS (phosphate buffered saline) ¿ Balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. ¿ Endotoxin and Mycoplasma tested ¿ Applications: immunological applications, such as Western Blotting. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ pH: 7.3 ¿ 7.5 ¿ Osmolality: 300¿5% mOsm/Kg H2O ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Alkali Scientific CellPro™ 1X DPBS, Dulbecco’s Phosphate Buffered Saline, without Calcium or Magnesium, Sterile Filtered, 500mL, 10/Case
Like PBS, DPBS is also a water-based solution containing sodium chloride and phosphate buffer that is used in biological applications. However, DPBS differs from PBS in that it also includes potassium chloride and is available in a larger variety of formulations.
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered) - 500ml
Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Teknova 1X PBS-MK with Sodium Chloride, 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Boston Bioproducts Inc Dulbecco's Phosphate Buffered Saline, DPBS (10X) - 1L
Dulbecco s Phosphate Buffered Saline DPBS (10X) - 1L (For Research Use and Further Manufacturing Only)
Promocell GmbH PromoCell Dulbeccos PBS pH 7,2-7,8 500 ml, Standard, with Ca++/ Mg++
Buffers and Salt Solutions are frequently used in tissue culture, cell isolation procedures, and molecular biology.For convenience, PromoCell offers ready-to-use Buffers and Salt Solutions. They are prepared from raw materials of the highest quality using tissue culture grade water.Dulbecco's PBS can be purchased with or without Ca^2 ^/ Mg^2 ^.
Teknova 10X PBS-MK, 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
LICOR PBS Blocking Buffer and PVDF Kit (Single buffer kit)
The non-mammalian Intercept Blocker in these kits is in PBS (phosphate-buffered saline) containing 0.06% Kathon II. The Immobilon-FL PVDF membrane included in these kits has been tested to ensure a low-background for infrared Western blot success. This kit contains Intercept (PBS) Blocking Buffer (500 mL) and Millipore Immobilon-FL PVDF Membrane (1 roll; 0.45µm, 26.5 cm x 3.75 m)
Alkali Scientific CellPro™ PBS 10X, pH 8.0, 1L
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Kd Medical Inc PBS BUFFER, 1X, PH 7.2, 500ML
1X PBS working solution commonly used in biological research. Used as a wash buffer in several research applications. One 500ml bottle.