Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 50 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660048.jpg-250.jpg)
Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 50 G
MES Monohydrate (2-(N-Morpholino) ethanesulfonic acid), 50g, is a biochemical reagent used for pH buffering in various laboratory applications. It is especially effective in maintaining a stable, non-interfering pH in experiments involving enzymes, proteins, and cell cultures. MES buffers provide consistency in biological assays, chromatography, and electrophoresis. This 50g volume is particularly suited for smaller scale applications or pilot studies where minimal reagent quantities are needed. It is ideal for laboratories that require precise pH control for their experiments without affecting the results of sensitive biochemical reactions or biological systems.
![Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661234.jpg-250.jpg)
Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 100 G
PIPES Sesquisodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sesquisodium salt) is a biochemical reagent supplied in a 100G package. It is used primarily for preparing buffered solutions in biological and chemical experiments. PIPES is highly stable across a broad pH range and is especially useful for maintaining pH in experiments involving biological systems such as enzymes, cells, and proteins. It is commonly utilized in electrophoresis, chromatography, and other biochemical processes where pH control is crucial. The sesquisodium form of PIPES ensures that solutions maintain consistent acidity or basicity without interference with the biological samples under study.
Alkali Scientific MOPSO Sodium Salt, 250 G
MOPSO Sodium Salt, 250 G, is a highly effective buffering reagent used in laboratory applications requiring stable pH control. MOPSO (Morpholinopropanesulfonic Acid Sodium Salt) is particularly useful for maintaining pH stability in experiments involving enzymes, proteins, and nucleic acids. This buffer offers a pH range of 6.5 to 7.9, making it ideal for biological assays, protein extraction, and other biochemical procedures. The 250 g size is suitable for labs that require frequent use of MOPSO buffer and offers a cost-effective solution for maintaining consistent conditions across a variety of experiments.
Medchemexpress LLC MOPSO sodium | 79803-73-9 | 97.0% | 247.24 | C7H14NNaO5S | 500 G
MOPSO sodium is the sodium salt of MOPSO, a zwitterionic buffering agent used in biochemical and molecular biology applications to prepare buffer solutions.
- Used for preparation of buffer solutions.
- Zwitterionic buffering agent; effective pH range typically near physiological pH.
- CAS number 79803-73-9.
- Chemical formula C7H14NNaO5S; molecular weight 247.24.
- Purity approximately 97.0% and available in 500 g packages.
Medchemexpress LLC Glycine, N-(2-amino-2-oxoethyl)-N-(carboxymethyl)-, sodium salt (1:2) | 41689-31-0 | 98.0% | 50 G
ADA disodium is a buffer salt derived from adenosine deaminase, commonly used in biochemical research for preparing enzyme buffers. This product is intended for research use only and is not for human or animal therapeutic use.
- Solid appearance
- White to off-white color
- Molecular weight: 234.12
- Chemical formula: C6H8N2Na2O5
- Solubility in water: 350 mg/mL
- Store at 4°C, sealed, away from moisture
- Store in solvent at -80°C for 6 months or -20°C for 1 month, sealed, away from moisture
Medchemexpress LLC PIPES (monosodium) | 10010-67-0 | 99.6% | 324.35 | 25 G
PIPES (1,4-Piperazinediethanesulfonic acid) monosodium is a pH buffer that can be mixed with another disodium salt form of PIPES. By changing the ratio and total amount of the PIPES monosodium and disodium buffers, the pH and ionic strength of the medium can be changed.
- pH buffer
- Can be mixed with disodium salt form of PIPES to adjust pH and ionic strength
- For research use only
Medchemexpress LLC Pipes (monosodium) | 10010-67-0 | 99.6% | 324.35 | 10 G
PIPES monosodium (1,4-Piperazinediethanesulfonic acid monosodium) is a pH buffer. It can be mixed with the disodium salt form of PIPES to adjust the pH and ionic strength of the medium by altering the ratio and total amounts of the buffers. This product is intended for research use only.
- Functions as a pH buffer
- Can be mixed with disodium salt form of PIPES
- Allows adjustment of pH and ionic strength
- Intended for research use only
Medchemexpress LLC HEPPSO | 68399-78-0 | 99.9% | 25 G
HEPPSO is a zwitterionic buffer designed for various research applications. It operates within a working pH range of 7.1-8.5 and exhibits a relatively high ability to bind copper(II). With a pKa of 7.84 at a 2.0 mM buffer concentration, it is suitable for use in laboratory settings.
- Zwitterionic buffer
- Working pH range of 7.1-8.5
- High ability to bind copper(II)
- pKa of 7.84 at 2.0 mM buffer concentration
- For research use only
Dojindo Molecular Technologies Inc BES (100G), Good's buffer, CAS: 10191-18-1, pka=7.15, Active pH 6.6-8, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
Medchemexpress LLC Mes (2-morpholinoethanesulphonic acid) | 4432-31-9 | 99.85% | 1 ML
MES (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer designed for various biochemistry and molecular biology applications. It functions effectively within a pH range of 5.5-7.0 and is widely recognized as a Good's buffer, suitable for experiments including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Buffer capacity from pH 5.5-7.0
- Ideal for cell culture, enzyme activity determination, electrophoresis, and protein studies
- Solid, white to off-white appearance
- Soluble in water and DMSO
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 6.5, 500 mL
Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications.The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information :pKa (25 °C) :7.1 (Free acid)Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.Appearance: LiquidPH Value: 6.5
Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 6.5, 100 mL
Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltpH: useful range: 6.0 - 7.2Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. PH Value: : 6.0 - 7.2